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pcgn n ras wt plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pcgn n ras wt plasmid
    Pcgn N Ras Wt Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+ras/pCGN+N-Ras+wt+(Plasmid+%2314723)/pm41597217-69-5-17
    Average 92 stars, based on 9 article reviews
    pcgn n ras wt plasmid - by Bioz Stars, 2026-11
    92/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Optimized conditions for GTP loading of Ras
    Article Snippet: Cancer mutations were introduced to the plasmid by QuikChange (Agilent). .. N-Ras (#25256) and K-RasB (#25153) isoforms were gifts from Cheryl Arrowsmith (Addgene plasmid #25256; http://n2t.net/addgene:25256 ; Research Resource Identifier: Addgene_25256; and Addgene plasmid #25153; http://n2t.net/addgene:25153 ; Research Resource Identifier: Addgene_25153). ..

    Article Title: Optimized conditions for GTP loading of Ras.
    Article Snippet: This region encodes the soluble G-domain of H-Ras without the hypervariable region, Cancer mutations were introduced to the plasmid by QuikChange (Agilent). .. N-Ras (#25256) and K-RasB (#25153) isoforms were gifts from Cheryl Arrowsmith (Addgene plasmid # 25256 ; http://n2t.net/addgene:25256 ; RRID:Addgene_25256; and Addgene plasmid # 25153 ; http://n2t.net/addgene:25153 ; RRID:Addgene_25153). ..

    Article Title: Optimized conditions for GTP loading of Ras
    Article Snippet: Cancer mutations were introduced to the plasmid by QuikChange (Agilent). .. N-Ras (#25256) and K-RasB (#25153) isoforms were gifts from Cheryl Arrowsmith (Addgene plasmid # 25256 ; http://n2t.net/addgene:25256 ; RRID:Addgene_25256; and Addgene plasmid # 25153 ; http://n2t.net/addgene:25153 ; RRID:Addgene_25153). ..



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    Image Search Results


    TIF‐IA protein accumulates in an animal model of OIS and in chronological and genetic models of ageing. (A) TIF‐IA and N‐ras immunohistochemistry were performed on liver sections from mice culled 6 days after hydrodynamic delivery of Nras G12V/D38A ( n = 5) or Nras G12V ( n = 5) transposons. Left: Representative images at 10× (scale bar 100 μm) and 40× (scale bar 20 μm) magnification. Right: QuPath software (v0.2.3) was used to quantify the percentage of TIF‐IA and N‐ras positive cells in five regions of interest (ROIs) per section. Pooled data (25 ROIs per condition) is shown. Mann–Whitney test determined significance. (B) Anti‐TIF‐IA immunohistochemistry was performed on colonic sections from young and old wild type (WT) and nfkb1 −/− mice ( n = 5 mice per condition). Left: Representative images at 10× and 40× magnification. Scale bar 20 μm. Right: QuPath software (v0.2.3) was used to quantify nuclear TIF‐IA intensity in five ROIs as above. Pooled data are shown. A Kruskal–Wallis test with Dunns multiple comparison was used to determine significance. ns, nonsignificant.

    Journal: Aging Cell

    Article Title: Loss of p62 Binding Allows TIF ‐ IA Accumulation in Senescence, Which Promotes Phenotypic Changes to Nucleoli and the Senescence Associated Secretory Phenotype

    doi: 10.1111/acel.70334

    Figure Lengend Snippet: TIF‐IA protein accumulates in an animal model of OIS and in chronological and genetic models of ageing. (A) TIF‐IA and N‐ras immunohistochemistry were performed on liver sections from mice culled 6 days after hydrodynamic delivery of Nras G12V/D38A ( n = 5) or Nras G12V ( n = 5) transposons. Left: Representative images at 10× (scale bar 100 μm) and 40× (scale bar 20 μm) magnification. Right: QuPath software (v0.2.3) was used to quantify the percentage of TIF‐IA and N‐ras positive cells in five regions of interest (ROIs) per section. Pooled data (25 ROIs per condition) is shown. Mann–Whitney test determined significance. (B) Anti‐TIF‐IA immunohistochemistry was performed on colonic sections from young and old wild type (WT) and nfkb1 −/− mice ( n = 5 mice per condition). Left: Representative images at 10× and 40× magnification. Scale bar 20 μm. Right: QuPath software (v0.2.3) was used to quantify nuclear TIF‐IA intensity in five ROIs as above. Pooled data are shown. A Kruskal–Wallis test with Dunns multiple comparison was used to determine significance. ns, nonsignificant.

    Article Snippet: Sections were incubated overnight at 4°C with the primary antibodies: rabbit RRN3 (Abcam 251933) and mouse NRas (Santa Cruz Biotechnology sc‐31), diluted 1:500 in antibody diluent (Dako).

    Techniques: Animal Model, Immunohistochemistry, Software, MANN-WHITNEY, Comparison